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Evolution of members of the genus Cardamine from the Anatolia-Caucasus region and the Balkan Peninsula
Kantor, Adam ; Slovák, Marek (advisor) ; Frajman, Božo (referee) ; Tribsch, Andreas (referee) ; Oberprieler, Christoph (referee)
This thesis aims to elucidate evolution of the members of the selected species groups of the genus Cardamine, which is a species-rich genus with cosmopolitan distribution and very complex evolutionary history. The studied species were represented by hygrophytic perennials, with the focus laid on their diversity in the Balkan Peninsula and Southwestern Asia, with the emphasis put on the Anatolica-Caucasus region. These regions harbour global biodiversity hotspots and important glacial refugia, yet, they have been largely understudied in terms of knowledge of evolutionary mechanisms and environmental factors that have played there a role of plants diversification and speciation drivers. In this thesis, questions concerning the phylogeny and taxonomy of the studied groups were addressed, with aim to determine the role of polyploidy and hybridization in their evolution. Hyb-Seq was the majorly applied method, representing a technique based on next-generation sequencing, which has proven to be a very efficient and versatile method for resolving evolutionary questions. An integrative approach was employed, combining Hyb-Seq with a variety of other karyological, molecular, cytogenetic and ecological niche modelling methods, which provided a very complex insight into the addressed topics. This thesis...
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Cardamine dentata, its distribution in Central Europe and relatinoship to C. pratensis
Holič, Filip ; Marhold, Karol (advisor) ; Chrtek, Jindřich (referee)
This thesis summarizes the documented information on the distribution of Cardamine dentata Schult. of the family Brassicaceae in the Czech Republic; it deals with the chromosome numbers and with ploidy levels of this species. Microsatellite markers were used to evaluate the potential relationships of C. dentata with other species of the group C. pratensis. Using a revision of available herbarium material it was found that the optimal conditions for the occurrence of C. dentata in the Czech Republic are in the phytogeographical regions of Thermophyticum and Mezophyticum at altitudes of 150-650 m a.s.l. No karyological data on this species were previously published from the area of the Czech Republic; using the flow cytometry and chromosomes counting it was revealed that in the area of the Czech Republic, populations with decaploid and undecaploid levels of ploidy occur. With help of microsatelite markers, it was confirmed that plants of C. dentata are genetically different from the other species of the C. pratensis group. Keywords: Cardamine, Cruciferae, microsatelite markers, chromosome numbers, geographical distribution, Czech Republic
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Cardamine dentata, its distribution in Central Europe and relatinoship to C. pratensis
Holič, Filip ; Marhold, Karol (advisor) ; Chrtek, Jindřich (referee)
This thesis summarizes the documented information on the distribution of Cardamine dentata Schult. of the family Brassicaceae in the Czech Republic; it deals with the chromosome numbers and with ploidy levels of this species. Microsatellite markers were used to evaluate the potential relationships of C. dentata with other species of the group C. pratensis. Using a revision of available herbarium material it was found that the optimal conditions for the occurrence of C. dentata in the Czech Republic are in the phytogeographical regions of Thermophyticum and Mezophyticum at altitudes of 150-650 m a.s.l. No karyological data on this species were previously published from the area of the Czech Republic; using the flow cytometry and chromosomes counting it was revealed that in the area of the Czech Republic, populations with decaploid and undecaploid levels of ploidy occur. With help of microsatelite markers, it was confirmed that plants of C. dentata are genetically different from the other species of the C. pratensis group. Keywords: Cardamine, Cruciferae, microsatelite markers, chromosome numbers, geographical distribution, Czech Republic
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